In Vivo Bioluminescence and Fluorescence Imaging

The Multi-User In Vivo Tracking Platform by Bioluminescence and Fluorescence, located on the ground floor of the UIPA building, has two devices specialized in imaging small animals, the IVIS™ Lumina Series II (In Vivo Imaging System, Perkin Elmer USA) and the IVIS™ Lumina XRMS (X-Ray Multi-species optical imaging System, Perkin Elmer USA). Both are capable of acquiring and quantifying luminescent and fluorescent images, thus allowing the path of pathogens or cells to be monitored when injected into whole animals, in addition to monitoring their intensity or viability longitudinally.

IVIS Lumina
IVIS XRMS

Technical specifications:

IVIS Lumina

  • 2D Acquisition
  • Bioluminescence Detection
  • Fluorescence Detection:
  • Excitation filters:
    – 430 nm
    – 465 nm
    – 500 nm
    – 535 nm
    – 570 nm
    – 605 nm
    – 640 nm
    – 675 nm
    – 710 nm
    – 745 nm
  • Emission filters:
    – GFP
    – DsRed
    – Cy5.5
    – ICG

IVIS XRMS

  • 2D Acquisition
  • X-ray Acquisition
  • Bioluminescence Detection
  • Fluorescence Detection:
  • Excitation filters:
    – 430 nm
    – 465 nm
    – 500 nm
    – 535 nm
    – 570 nm
    – 605 nm
    – 640 nm
    – 675 nm
    – 710 nm
    – 745 nm
  • Emission filters:
    – GFP
    – DsRed
    – Cy5.5
    – ICG
3rd CENABIO’s meeting: Basic Basic principles of Cell and Animal Imaging by Bioluminescence and Fluorescence (original video in portuguese)
Isalira Ramos
Technical manager
CV

Contact: plataformaivis@cenabio.ufrj.br

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